PNIBLOG

Stem cell therapy and brain health

Clinical trials using stem cells have shown promising early results as cellular therapies in regenerative medicine, as well as in potential anti-cancer applications.

The use of stem cells as medical treatments is already having an impact on patients in the clinic, according to early results from new studies being led by Pacific Neuroscience Institute (PNI) investigators. The flexibility and adaptability of these cells make them especially exciting new therapeutic options, both for rebuilding damaged brain tissues as well as for combatting cancer.

We sat down with expert neurosurgeon Achal Singh Achrol, MD, Director of Neurosurgery Clinical Trials at PNI, to discuss ongoing stem cell studies at PNI and John Wayne Cancer Institute (JWCI), and the implications of regenerative medicine in the future as ­cellular therapy in neurological diseases.

Exploring Stem Cells as Treatments

Achal Singh Achrol, MD

Achal Singh Achrol, MD

Stem cells have the unique ability to continuously replenish themselves, called “self-renewal”, as well as develop into many different types of cells that make up our body (called “differentiation”), such as muscle cells, blood cells, or brain cells.

“The goal of stem cells in the body is essentially to maintain homeostasis – which is basically the status quo functioning of our body – by regenerating and replenishing cells over time, such as in the skin or gut,” said Dr. Achrol. “Since they play a key role in injury recovery and healing, we’re using this property of stem cells to boost healing in areas where patients’ own stem cells haven’t been successful enough at their baseline abilities of wound healing.”

The goal of the clinical trials program at PNI and JWCI is to address unmet medical needs in our neuroscience patient populations, and support the translation of new therapeutic innovations that improve the quality of lives for our patients.

Daniel Kelly, MD and Achal Singh Achrol, MDDr. Achrol is the principal investigator for two recently completed neurosurgical trials to assess the safety and efficacy of stem cells to promote motor recovery in patients with different forms of brain injury, including ischemic stroke and traumatic brain injury (TBI).

“The brain is in a protected environment surrounded by what’s called the blood brain barrier, protecting it from exposure to toxins, or immune cells that might cause inflammation and harm,” observed Dr. Achrol. “But the blood brain barrier also prevents our immune cells and stem cells from getting in when they need to help fight cancer or help promote recovery of the brain after an injury, like a stroke or concussion.”

Using a highly precise, minimally invasive neurosurgical approach, Dr. Achrol placed modified stem cells directly into the site of brain injury in stroke and TBI participants. “The goal of cellular therapy is to get those stem cells to the areas that they can’t get to naturally, and minimally invasive neurosurgery is a tool for us to deliver these cells exactly where they need to be in the right quantities needed to have an impact.”

Both studies were multi-center, international collaborations, with PNI/JWCI leading the overall surgical enrollment worldwide in the ischemic stroke trial. While the latest Phase 2B stroke study results have not yet been published, Dr. Achrol is hopeful that the findings will be as positive as earlier Phase 1/2A data that showed the stem cells were safe and associated with significant motor recovery in patients with chronic strokes.

As the fastest-enrolling surgical site for the TBI study, PNI investigators have seen early results that are very encouraging in the recently completed interim analysis. “This global, randomized clinical trial is very exciting as it is the largest stem cell trial ever conducted for TBI,” said Dr. Achrol. “The interim analysis results show significant improvement of patients treated with stem cells versus the controls, with no safety concerns, which is a significant milestone for regenerative medicine in brain health.”

“Beyond these particular ischemic stroke and traumatic brain injury trials, just the fact that we are delivering stem cells as surgical treatments in the brain with collaborative groups that span the world is really a landmark time in the field. It’s very exciting, both for us as clinicians, and for our patients as well.”

What’s Next?

“Pending the Phase 2 trials, the next steps would be to launch the larger Phase 3 trials to finalize FDA approval of these stem cell therapies in these indications,” Dr. Achrol commented. “We also hope to see extended indications in other forms of injury to the central nervous system. Examples of this include spinal cord injury, intracerebral hemorrhage (for example when patients have high blood pressure injury to the brain), and neurodegenerative diseases such as ALS and dementia. Using stem cell approaches to boost brain function and prevent effects of the aging brain is of interest in maintaining brain health as well.”

Use of Stem Cells in Regenerative Medicine

“Because of the blood brain barrier, the minimally invasive neurosurgical approach is ideal for these clinical trials, as you know exactly where the cellular therapy is being delivered and how much is actually at the target site. While it may be possible to offer these therapies less invasively in the future, for example by IV, there is more to work out there in terms of how much will actually get to the target site in the brain, and the effects of exposure of other organs to the cells given systemically,” said Dr. Achrol. “We already have FDA–approved neurodevices like Deep Brain Stimulation (DBS) for Parkinson’s disease where we place electrodes in specific regions of the brain to increase the activity of cells and promote their function. So surgically placing something biological and not electrical to stimulate cells and promote brain function, is not too conceptually different from existing treatment workflows. Stimulating devices in the brain are similar to devices elsewhere, like cardiac pacemakers, which are all just electrically telling cells to activate. Newly introduced treatment paradigms with stem cells would be conceptually similar, but relying on a biological tool rather than a device to help promote and boost the function of cells at the site of dysfunction.”

For clinical usage in the future, it may become feasible to deliver stem cells less invasively via IV, potentially by genetically engineering the cells so that they are programmed to get to their target site more efficiently.

Autologous vs. Allogenic Stem Cells

Autologous vs Allogenic stem cells

Autologous stem cells are those that are taken from a patient, boosted, and returned to the same patient. Allogenic stem cells are taken from a donor, and conceptually similar to a blood transfusion. There are advantages and disadvantages to each type of cell and studies are being conducted using both. In either case, the stem cells are often modified to help target and boost their activity during treatment and beyond. In a recently published paper in Nature, autologous stem cells acted as an unlimited source of patient-specific cell repair.

Autologous vs Allogenic Stem Cells - advantages and disadvantages

The Sanbio stem cell line consists of cells that are modified to release trophic and growth factors for wound healing, so that they modulate the immune system to heal rather than being inflamed. The stem cells are linked to the notch-1 protein which specifically helps target healing in neural cells.

“The allogenic mesenchymal stem cells we used in the Sanbio trials can be viewed as wound healing helpers, like a traffic cop directing signals to promote a healing environment. If they are eventually recognized by the immune system as foreign cells, and are cleared out from the body as a result, they would still have started a process of healing that could have a lasting mechanism of action,” Dr. Achrol observed. “In the future, it is possible that allogenic cells from a standardized donation bank can be antigen-matched similar to how we do for blood banks to limit these immune responses. There is also exciting new data with genetically-engineering the donor cells to prevent immune responses toward them. In the current allogenic trials the cells presumably initiate healing cascades giving the body’s own cells the capacity for healing with longer lasting effect. The hope is that by limiting or preventing immune clearance of the donor cells we can get even more pronounced recovery.”

Eventually using advanced technologies that are already available, autologous stem cells could be genetically edited so that they don’t ever get cleared from the body but become integrated and help rebuild damaged tissues. It is not too difficult to imagine that these could be used in a similar manner to organ transplants. For example, if the stem cells were brain-specific neural stem cells, those that generate neurons in the brain, they could regrow and rebuild areas of brain tissue left damaged by injury.

Stem Cells as Anti-Cancer Agents

Another aspect of stem cells is their potential as anti-cancer therapies. Dr. Achrol explained, “We take that stem cell that can regenerate – healing strokes and spinal cord injuries – but rather than using it as a growth promoting agent, we can use it as a factory to create cells in the lab. We can create a line of stem cells that are then differentiated into specific types of immune cells, such as engineered T-cells and anti-cancer myeloid cells, which can be used to specifically seek out and kill cancer cells.”

A similar technology to create anti-cancer T-cells is being used in clinical trials sponsored by our Santa Monica neighbors, Gilead’s Kite. “With the proof of principal provided by studies at Gilead/Kite, there is a great deal of excitement for these cellular therapy approaches in cancer,” Dr. Achrol added.

“This has relevance to us as a continuum of leading-edge research at PNI and JWCI. JWCI’s Cellular Therapy Facility has been used for several years to manufacture anti-cancer T-cells to treat patients with melanoma. We are now building on this resource to support studies with stem cells in stroke, TBI, and dementia and developing the capacity to combine both these arms of research to create a stem cell-based, personalized medicine workflow for engineering anti-cancer immune cells. In this way, the same Cellular Therapy Facility is supporting treatments not only to help the brain recover from injury, but also to develop anti-cancer immune cells to fight cancers in the brain, like glioblastoma and brain metastases.”

A Look Into the Future

Cellular therapies for regenerative medicine and anti-cancer treatments are already reaching patients through advanced clinical trials, and future treatment options are knocking at the door.

clinical trials

Source: Everyday Health

“An exciting new area of rational new drug design involves the development of patient-specific cell lines for guiding drug development and treatment selection,” stated Dr. Achrol. One such scenario might include taking a small pinch of skin cells from a patient that could be reprogrammed into stem cells and grown out to create any target cell of interest, such as neural cells in a petri dish.

Dr. Achrol concluded, “Diseased neurons in patients with a particular disease like ALS could be compared to healthy neurons on a molecular level. The patient’s disease can be modeled, and drugs created to address the anomalies, all in the dish. Even toxicity studies could be conducted in the lab by testing against cardiac or liver cells made out of the same stem cell bank, ensuring safety before ever initiating a trial in the patient. Such a new paradigm in clinical trial design could help us do drug development faster, and could minimize side effects and the type of toxicity events in clinical trials that can otherwise prevent therapies from making it through to the patients who could really benefit from them.”

For more information about neurosurgical clinical trials at Pacific Neuroscience Institute, contact Dr. Achrol’s clinics at 310-582-7099.

Related articles:

Reversing the effects of stroke

Can stem cells repair traumatic brain injury?

 

Zara Jethani

Zara Jethani, MS, MBA, is the marketing and communications director for Pacific Neuroscience Institute. Her background is in graphic design, molecular genetics research and healthcare marketing.